Risk Factors Associated with antimicrobial Residues in the Milk Consumed in Nyala, South Darfur State, Sudan

Fathalrhman E. Addoma, Atif E. Abdelgadir, Ayman El Nahas, Ibtisam E. El Zubeir

Abstract


This study aimed to assess the contamination of milk by antimicrobial residues consumed in Nyala, South Darfur State, Sudan. A questionnaire and direct interviews with veterinarians at the veterinary pharmacies (n= 30) as well as with dairy farmers (n= 30) were carried out to determine the various risk factors associated with the presence of antimicrobial residues in milk. In addition, hundred milk samples were collected from dairy farms and sale points (n= 50, each) and were analyzed for antimicrobial residues by using Trisensor kit. The results revealed that 73.3% of the veterinarians believe that prescriptions are not necessary for drug dispensing, and that 43.3% of the veterinarians practice whole sale of antibiotics to dairy farm owners (P<0.001). However, 60% of the veterinarians guide the dairy farmers to be strict to antibiotic dose and 73.3% advise them about the way of administration. Moreover, only 56.7% of the veterinarians advise the dairy farmers about the importance of the withdrawal period, and 93.3% stated that most of the saleable drugs are broad spectrum antibiotics. On the other hand, 16.7% of the dairy farmers add antibacterial drugs to animal feed, with significant difference (P>0.01) between south and north Nyala. Fifty percent of dairy farmers administer antibiotics to healthy animal as prophylactic therapy, only 26.7% of them consult the veterinarians, 70% keep drug in the cattle yard and treat the animals by themselves, 73.3% do not apply the withdrawal period and non of them have balances to measure animal weight for suitable drug dosage. Furthermore, one hundred raw and heated milk samples (50 from dairy farms and 50 from sale points) collected by non- probability sampling method were screening for contamination with drugs residues using the Trisensor kit (detects simultaneously beta-lactams, tetracyclines and sulfonamides, in addition to the Triaminosensor kit (detects simultaneously gentamicin, streptomycin and neomycin). In both raw and heated milk, high contamination rates with tetracyclines (34% and 31%, respectively) and sulfonamides (31% and 29%, respectively) were detected, but with no significant difference (P>0.05). Contamination with beta-lactams was higher in milk samples collected from the sale points (37%) than in the samples from dairy farms (32%), but also the difference was not significant (P>0.05). While, neomycin was not detected, gentamicin was detected in 25% and 32% and streptomycin was found in 19% and 26% of raw and heated milk, respectively.
In conclusion, contamination of milk by antimicrobial in Nyala indicates lack of proper and direct veterinary supervision in implementation of the legislations on the presence of veterinary drugs residues in milk in addition to the ignorance of withdrawal period of drugs.

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References


Abbasi, M.M.; Babaei, H.; Ansarin, M.; Nourdadgar, A.O.S. and Nemati, M. (2011). Simultaneous determination of tetracyclines residues in bovine milk samples by solid phase extraction and HPLC-FL method. Advanced Pharmaceutical Bulletin, 1(1): 34-39.

Adrian, J.; Pasche, S.; Diserens, J.; Snchez-Baeza, F.; Gaod, H.; Marco, M.P. and Voirin, G. (2009). Waveguide interrogated optical immunosensor (WIOS) for detection of sulfonamide antibiotics in milk. Biosensors and Bioelectronics, 24: 3340–3346.

Al Zuheir, I.M. (2012). Detection of beta-lactams and tetracyclines antimicrobial residues in raw dairy milk for human consumption in Palestine. Walailak J. Sci.and Tech., 9 (3): 277‐279.

Alanis, A.J. (2005). Resistance to antibiotics: Are we in the post antibiotic era. Archives of the Medical Research, 36: 697-705.

Aning, K.G.; Donkor, E.S.; Omore, A.; Nurah, G.K.; Osafo, E.L.K. and Staal, S. (2007). Risk of exposure to marketed milk with antimicrobial drug residues in Ghana. The Open Food Science Journal, 1: 1-5.

Aryal, S. (2001). Antibiotic resistance: A concern to veterinary and human medicine. Nepal Agric. Res. J., 5: 66-69.

Barrow, G.I. and Feltham, R.K (1993), Cowan and Steel Manual for the Identification for Medical Bacteria, 3rd ed., Cambridge.

Chenggang, J.; Adrian, E.; Lijie, F. and Xiaoyun, L. (2011). Framing a global health risk from the bottom-up: User perceptions and practices around antibiotics in four villages in China. Health Risk and Society, 13 (5): 433-449.

Darwish, W.S.; Eldaly, E.A.; El-Abbasy, M.T.; Ikenaka, Y.; Nakayama, S. and Ishizuka, M. (2013). Antibiotic residues in food: The African scenario. Japanese Journal of Veterinary Research, 61: 13-22.

Edima, H.; Tofaing, J.T.; Ngoune, L.T. and Ndjouenkeu, R. (2012). Assessment of antibiotic residues in cow milk produced in Ngaoundere. International Journal of Biology, Pharmacy and Applied Sciences, 1 (11): 1530-1538.

El Zubeir, I.E.M. and El Owni, O.A.O. (2009). Antimicrobial resistance of bacteria associated with raw milk contaminated by chemical preservatives. World J. Dairy and Food Sci., 4 (1): 65-69.

El Zubeir, I.E.M.; Kiessling, S.; Kutzer, P.; Wieler, L.H. and El Owni, O.A.O. (2012). Characterization of tetracycline resistant Staphylococcus aureus isolated from bovine

+ve streptomycin

-ve neomycin

-ve streptomycin

-ve neomycin

+ve gentamicin

Control line

+ve gentamicin

Control line

-ve neomycin

+ve streptomycin

-ve gentamicin

Control line

U. of K. J. Vet. Med. Anim. Prod., Vol. 7, Issue 1 (2016) p 22-32

ISSN: 24345

mastitis in Khartoum State, Sudan. University of Khart. J. Vet. Med. and Anim. Prod., 3 (2): 53-64.

El Zubeir, I.E.M. and Mahala, A.G. (2011). An overview of the management practices and constrains at the dairy camps in Khartoum State, Sudan. Research Opinions in Animal and Veterinary Sciences, 1 (7): 425-428.

Kaneene, J. and Ahl, A. (1987). Drug residues in dairy cattle industry: epidemiological evaluation of factors influencing their occurrence. J. Dairy Sci., 70: 2176- 2180.

Katakweba, A.A.S.; Mtambo, M.M.A.; Olsen, J.E. and Muhairwa, A.P. (2012). Awareness of human health risks associated with the use of antibiotics among livestock keepers and factors that contribute to selection of antibiotic resistance bacteria within livestock in Tanzania. Livestock Research for Rural Development, 24 (10) http://www.lrrd.org/ lrrd/kata24170.htm

Kirbis, A. (2006). Microbiological 5-plate screening method for detection of tetracyclins, aminoglycosides, cephalosporins and macrolides in milk. Slov. Vet. Res., 43 (4): 161-168.

Komolafe, O.O. (2003). Antibiotic resistance in bacteria- an emerging public health problem. Malawi Medical Journal, 15 (2): 63- 67.

Kukanich, B.; Gehring, R.; Webb, A.I.; Craigmill, A.L. and Riviere, J.E. (2005). Effect of formulation and route of administration on tissue residues and withdrawal times. J. Am. Vet. Med. Assoc.. 227: 1574-1577.

Movassagh, M.H. (2011). Study of antibiotics residues in cow raw milk by copan milk test in Parsabad region, Ardabil Province, Iran. Annals of Biological Research, 2 (4): 355-359.

Okeke, I.N. and Ojo, K.K. (2010), Antimicrobial Resistance and Use in Africa. In: Sosa, A.de J.; Byarugaba, D.K.; Ama´ bile-Cuevas, C.F.; Hsueh, P.R.; Kariuki, S. and Okeke, I.N. (Editors). Antimicrobial Resistance in Developing Countries, US Springer Science and Business Media, pp 301-314.

Reybroeck, W. and Ooghe, S. (2012). Validation of post-screening tests applied in the procedure of the interprofessional organizations for the screening of antimicrobials in milk. In: Validation of post-screening tests applied in the procedure of the interprofessional organizations for the screening of antimicrobials in milk. Report p. 1-27.

Ryoba, R.; Kweka, L.; Kazwala, R. and Kurwijila, L.R. (2007). Assessment of antibiotic residues in milk collected at Tanga dairy cooperative union centre in Tanga municipality, Tanzania. Tanzania Veterinary Journal, 24 (1): 38-51.

Said Ahmad, A.M.M.; El Zubeir, I.E.M.; El Owni, O.A.O. and Ahmed, M.K.A. (2008). Assessment of microbial loads and antibiotic residues in milk supply in Khartoum State, Sudan. Research Journal of Dairy Sciences, 2: 57-62.

Salman, A.M.; El Nasri, H.A. and Osman, I.A.M. (2012). Detection of antibiotic residues in milk using Delvotest kit and the disc assay methods in Khartoum state, Sudan. University of Khartoum J. Vet. Med. and Anim. Prod., 3(2): 3-15.

Sawant, A.A.; Sordillo, L. M. and Jayarao, B.M. (2005). A survey on antibiotic usage in dairy herds in Pennsylvania. J. Dairy Sci., 88: 2991–2999.

Shitandi, A.A. and Sternesjö, A. (2004). Factors contributing to the occurrence of antimicrobial drug residues in Kenyan milk. J. Food Prot., 67 (2): 399-402.

Stolker, A.A.M. and Brinkman, U.A.T.H. (2005). Analytical strategies for residue analysis of veterinary drugs and growth promoting agents in food producing animals- a review. J. Chromatogr. A, 1067: 15-53.

Stolker, A.A.M.; Zuidema, T. and Nielen, M.W.F. (2007). Residue analysis of veterinary drugs and growth-promoting agents. Trends in Analytical Chemistry, 26: 967-979

Thrusfield, M. (2007). Veterinary Epidemiology. 3th edition p; 189 and p: 230.


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