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The effect of entomopathogenic fungi Metarhizium anisopliae var acridum and Beauveria bassiana on the survival of mosquito larvae of Anopheles arabiensis Patton and Culex quinquefasciatus Say

Asmaa El Abas El Agali, El Amin El Rayah Mohammed, Abd El Wahab Hassan

Abstract


Because of environmental considerations and the development of resistance in vectors, efforts had been directed to microbial control. Immature stages of mosquito are useful components of malaria control program. The entomopathogenic hyphomycetous fungi Metarhizium anisopliae var acridum and Beauveria bassiana were selected  and tested against early (L1-2) and late (L3-4) stages of mosquito larvae Anopheles arabiensis and Culex quinquefasciatus. Two concentrations of each fungual spores were used to perform the laboratory bioassays and time to death was determined for each mosquito species.

 Metarhizium anisopliae var acridum and Beauveria bassiana caused high mortality in An. arabiensis and C. quinquefasciatus. However, B. bassiana was less effective compared to M. anisopliae var acridum.

Both An. arabiensis and C. quinquefasciatus were susceptible to the two fungi concentrations at early stage. On the other hand, the older one were less susceptible.

This research showed the potentiality of the two fungal species against mosquitoes in the larval stage, and the mortality percentage depends on fungus species itself and larval stage targeted. The increase in  fungus concentration did not showed a proportional increase in mortality, may be due to the spores clumped.


Keywords


Moequito biocontrol;Entomopathogenic Fungi;Metarhizium anisopliae;Beauveria bassiana

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References


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